Effect of Low Frequency Low-Level Infra-Red Diode Laser Therapy on Third Degree Burn Healing in Rats Skin

Purpose: The aim of present investigation was to evaluate low frequency low-level infra red diode laser therapy for a third degree burn healing in rats skin.Materials and Methods: 36 rats were divided into groups one and two. On day zero three third degree burns were made on the dorsum of each rat by steam. In group one ,first burn were exposed to a 80 Hz –pulsed 890 nm infra red diode laser with o.396 J/cm2 three times a week. In group two, first burn was exposed to the laser with shot down laser. In both groups, second burns were treated with topical application of nitrofurazone % 0.2. In both groups, third burns did not receive any treatment and were considered as control burn. Burns were clinically examined .Wound closure of burns were recorded and compared by paired student t test .On days 8, 12, and 20 microbiological samples of burns were collected and analysed. The data of each extracted micro organisms of burn were compared. A further comparison was made between micro organisms assumed to be pathogen and micro organisms presumed to be non-pathogen. Data were analyzed by chi-square method.Results: Paired student t test showed wound closure rate of laser-treated burns (17.6±days) was significantly higher than that of its control burn(19.6±days)(p=0.007). There were not significant difference between each micro organism and also between micro organisms assumed to be pathogen and micro organisms presumed to be non-pathogen.Conclusion: Low-level laser therapy with a diode laser significantly accelerated third degree burn healing in rat.